diff --git a/tests/SEGHydrationDeleteAndReload.spec.ts b/tests/SEGHydrationDeleteAndReload.spec.ts
new file mode 100644
index 000000000..8a1701bad
--- /dev/null
+++ b/tests/SEGHydrationDeleteAndReload.spec.ts
@@ -0,0 +1,67 @@
+import {
+  checkForScreenshot,
+  expect,
+  screenShotPaths,
+  test,
+  visitStudy,
+  waitForViewportRenderCycle,
+} from './utils';
+
+test.beforeEach(async ({ page }) => {
+  const studyInstanceUID = '1.3.6.1.4.1.14519.5.2.1.256467663913010332776401703474716742458';
+  const mode = 'segmentation';
+  await visitStudy(page, studyInstanceUID, mode, 2000);
+});
+
+test('should fully remove segmentation overlay after repeated load-and-delete cycle', async ({
+  page,
+  DOMOverlayPageObject,
+  leftPanelPageObject,
+  rightPanelPageObject,
+  viewportPageObject,
+}) => {
+  const activeViewport = await viewportPageObject.active;
+
+  await leftPanelPageObject.loadSeriesByModality('SEG');
+
+  await expect(DOMOverlayPageObject.viewport.segmentationHydration.locator).toBeVisible();
+
+  let viewportRenderCycle = waitForViewportRenderCycle(page);
+  await DOMOverlayPageObject.viewport.segmentationHydration.yes.click();
+  await viewportRenderCycle;
+
+  viewportRenderCycle = waitForViewportRenderCycle(page);
+  await rightPanelPageObject.labelMapSegmentationPanel.panel.moreMenu.delete();
+  await viewportRenderCycle;
+
+  expect(await rightPanelPageObject.labelMapSegmentationPanel.panel.getSegmentCount()).toBe(0);
+
+  // reload SEG series
+  viewportRenderCycle = waitForViewportRenderCycle(page);
+  await leftPanelPageObject.loadSeriesByModality('SEG');
+  await viewportRenderCycle;
+
+  await expect(DOMOverlayPageObject.viewport.segmentationHydration.locator).toBeVisible();
+
+  viewportRenderCycle = waitForViewportRenderCycle(page);
+  await DOMOverlayPageObject.viewport.segmentationHydration.yes.click();
+  await viewportRenderCycle;
+
+  await checkForScreenshot(
+    page,
+    activeViewport.pane,
+    screenShotPaths.segHydrationDeleteAndReload.viewportAfterSecondHydration
+  );
+
+  viewportRenderCycle = waitForViewportRenderCycle(page);
+  await rightPanelPageObject.labelMapSegmentationPanel.panel.moreMenu.delete();
+  await viewportRenderCycle;
+
+  expect(await rightPanelPageObject.labelMapSegmentationPanel.panel.getSegmentCount()).toBe(0);
+
+  await checkForScreenshot(
+    page,
+    activeViewport.pane,
+    screenShotPaths.segHydrationDeleteAndReload.viewportAfterSecondDelete
+  );
+});
diff --git a/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondDelete.png b/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondDelete.png
new file mode 100644
index 000000000..0cb3b0358
Binary files /dev/null and b/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondDelete.png differ
diff --git a/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondHydration.png b/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondHydration.png
new file mode 100644
index 000000000..c4a232756
Binary files /dev/null and b/tests/screenshots/chromium/SEGHydrationDeleteAndReload.spec.ts/viewportAfterSecondHydration.png differ
diff --git a/tests/utils/screenShotPaths.ts b/tests/utils/screenShotPaths.ts
index bdab7e700..73cce0481 100644
--- a/tests/utils/screenShotPaths.ts
+++ b/tests/utils/screenShotPaths.ts
@@ -132,6 +132,10 @@ const screenShotPaths = {
     segPreHydration: 'segPreHydration.png',
     segJumpToSegment: 'segJumpToSegment.png',
   },
+  segHydrationDeleteAndReload: {
+    viewportAfterSecondHydration: 'viewportAfterSecondHydration.png',
+    viewportAfterSecondDelete: 'viewportAfterSecondDelete.png',
+  },
   segHydrationThenMPR: {
     segPostHydration: 'segPostHydration.png',
     segPostHydrationMPRAxialPrimary: 'segPostHydrationMPRAxialPrimary.png',